Coding

Part:BBa_K4949003

Designed by: Umanitoba 2023   Group: iGEM23_UManitoba   (2023-10-10)

GEN0105

GEN0105 is a PLA depolymerase originally identified in Paenibacillus amylolyticus, strain TB-13. This strain has been observed to degrade a variety of aliphatic polyesters, including PLA plastics with maximal activity occurring in the range of 45-55°C (Hajighasemi et al. 2016).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 418
    Illegal NgoMIV site found at 505
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference: Hajighasemi, M., Nocek, B. P., Tchigvintsev, A., Brown, G., Flick, R., Xu, X., Cui, H., Hai, T., Joachimiak, A., Golyshin, P. N., Savchenko, A., Edwards, E. A., & Yakunin, A. F. (2016). Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases. Biomacromolecules, 17(6), 2027–2039. https://doi.org/10.1021/acs.biomac.6b00223

[edit]
Categories
Parameters
None